Chromatographic separation plays a vital role in various areas, as this technique can deliver high-quality products both in lab- and industrial-scale processes. Economical and also ecological benefits can be expected when optimizing such processes with mathematical methods. However, even small perturbations in the operating conditions can result in significantly altered results, which may lead to … Read more
Column liquid chromatography is an important technique applied in the production of biopharmaceuticals, specifically for the separation of biological macromolecules such as proteins. When setting up process conditions, it is crucial that the purity of the product is sufficiently high, even in the presence of perturbations in the process conditions, e.g., altered buffer salt concentrations. … Read more
Mathematical modeling, simulation, and optimization can significantly support the development and characterization of chromatography steps in the biopharmaceutical industry. Particularly mechanistic models become preferably used, as these models, once carefully calibrated, can be employed for a reliable optimization. However, model calibration is a difficult task in this context due to high correlations between parameters, highly … Read more